|Researchers annotate mouse microarray used in studies of early development|
Edward R. Winstead
May 28, 2001
Researchers at the US National Institute on Aging have annotated a 15,000-gene mouse microarray used in more than 100 laboratories to profile gene expression during early development. They classified 4,000 genes in categories such as 'cell cycle,' 'DNA replication' and 'energy/metabolism' based on their sequence similarity to known genes in public databases. In addition, the researchers re-sequenced the set of cDNA clones to improve the data.
"Based on nucleotide and protein matches, we have done a preliminary functional classification of 4,027 genes," the researchers write this month in Nature Genetics. Minoru S.H. Ko, head of the institute's Developmental Genomics and Aging Section, Laboratory of Genetics in Baltimore, led the study. His group developed the microarray in May 2000 using a library of mouse cDNA clones constructed over the last decade.
About half the genes on the microarray resemble known genes in public databases. "Because fewer than 10,000 known mammalian genes are in the public database, we infer that up to 75 percent of those are included in this set," the researchers say.
The mouse microarray is known as the NIA 15K cDNA clone set. In one of the first experimental uses of the microarray, Ko's team last year characterized gene expression in mouse placental and embryonic tissues halfway through gestation.
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