Circular representation of the Thermoanaerobacter tengcongensis genome. Circles display (from the outside): (1) Physical map scaled in megabases from base 1, the start of the putative replication origin. (2) Coding sequences transcribed in the clockwise direction. (3) Coding sequences transcribed in the counterclockwise direction. (4) G + C percent content (in a 10-kb window and 1-kb incremental shift); values >37.6% (average) are in red and smaller in blue. (5) GC skew (G-C/G + C, in a 10-kb window and 1-kb incremental shift); values greater than zero are in magenta and smaller in green. (6) Repeated sequences; short 30-bp repeats are in red and other types in blue. (7) tRNA genes. (8) rRNA genes. Genes displayed in 2 and 3 are color-coded according to different functional categories: translation/ribosome structure/biogenesis, pink; transcription, olive drab; DNA replication/recombination/repair, forest green; cell division/chromosome partitioning, light blue; posttranslational modification/protein turnover/chaperones, purple; cell envelope biogenesis/outer membrane, red; cell motility/secretion, plum; inorganic ion transport/metabolism, dark sea green; signal transduction mechanisms, medium purple; energy production/conversion, dark olive green; carbohydrate transport/metabolism, gold; amino acid transport/metabolism, yellow; nucleotide transport/metabolism, orange; coenzyme metabolism, tan; lipid metabolism, salmon; secondary metabolites biosynthesis/transport/catabolism, light green; general function prediction only, dark blue; conserved hypothetical, medium blue; hypothetical, black; unclassified, light blue; pseudogenes, gray. (b) Linear representation of the T. tengcongensis genome. Genes are color-coded according to different functional categories as described above for a , with above character-string representing gene names or IDs. Arrows indicate the direction of transcription. Genes with authentic frameshift and point mutations are indicated with X. Paralogous gene families are indicated by family ID in a box above the predicted genes. Numbers next to GES (Goldman-Engleman-Steitz) represent the number of membrane-spanning domains predicted by Goldman-Engleman-Steitz scale calculated by TMHMM. Proteins with five or more GES are indicated. The 305 copies of the 30-bp short repeat, clustered in two regions, are indicated with the greater-than symbol. RNA genes, including those of rRNA, tRNA, and other RNA genes, signal peptides and long repeats are also indicated. Numbers on the tRNA symbols represent the number of tRNAs in the cluster.

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