|Born to Run|
|Idaho Gem, the First Cloned Mule|
|By Nancy Touchette
May 30, 2003
Much to the delight of mule-racing enthusiasts throughout the world, Idaho Gem, the first mule ever cloned, was born on May 4, 2003, in Moscow, Idaho. At twelve minutes of age, the mule stood up and started to buck as he was still being dried off.
“He’s got personality,” says Gordon L. Woods of the University of Idaho, who led the cloning project. “When he was 33-hours old, he darted around on the grass like a little rabbit. He hasn’t missed a beat since.”
Idaho Gem was cloned from the brother of a world-champion racing mule. Mules are sterile and unable to pass on their genes, but cloning offers a means of propagating promising bloodlines.
The birth of Idaho Gem grew out of attempts to understand horse fertility. Despite the success of in vitro fertilization in humans, cattle, and other species, only two foals have ever been born as a result of the technique. Attempts to clone horses have also met with failure.
While trying to understand the cloning problems, Woods noticed that many horse cells have less calcium than normal. Because calcium regulates many cell activities, he and his colleagues decided to increase the calcium in eggs for cloning.
As the horse project was getting underway, Don Jacklin, president of the American Mule Racing Association, offered funding on the condition that the researchers first clone a mule.
“We wanted to do something no one else had done,” says Jacklin. “And for the first equine clone, we didn’t want to create a monster that would overpopulate the world. That’s not a problem with a sterile hybrid.”
Mules result from the breeding of a male donkey with a female horse. Donkeys have 62 chromosomes, horses have 64, and mules have 63. Mules are sterile because their chromosomes cannot pair up evenly, and as a result they do not produce eggs and sperm.
To create Idaho Gem, the researchers used fetal cells from a brother of Taz, the champion racer owned by Jacklin. In a process known as nuclear transfer, they inserted the mule DNA into a horse egg cell that contained no genetic material. The resulting cloned embryo was transplanted into the uterus of a surrogate mare.
The project is reported today online in Science. Two other cloning attempts have been successful, and the mules are expected be born this summer.
The researchers are now trying to clone a horse using the same strategy. It is unclear what impact the technology could have on equine sports.
The Jockey Club, which regulates the thoroughbred horse racing industry, strictly forbids in vitro fertilization and cloning. But sport horses used for other disciplines, including Olympic show jumping, are under no such restrictions.
The American Mule Racing Association does not restrict any kind of reproductive technology.
“I hope one of these clones will be made available for racing,” says Jacklin. “I see a whole revolution in terms of the way breeding is done.”
Woods believes the horse research could have implications for human medicine. Understanding how calcium is regulated in horse cells could help researchers studying diseases like diabetes and prostate cancer, which involve high calcium levels.
Furthermore, horses may be good models of diseases that occur late in life because horses—unlike laboratory mice—can live for 30 years or more.
“The horse is a thousand-pound mouse that kicks,” Woods says.
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