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How to query the Homophila database. (TOP) Schematic of a Homophila
query. The user enters the text query in the form of human disease name,
Online Mendelian Inheritance in Man (OMIM) number, fly gene name, or keyword
search through the human disease entry box. The database then opens a
window with information on the disease name, and human and fly genes that
match the key word query. The user then can examine the details of an
individual human to Drosophila BLAST comparison
to get more information on the specific BLAST score,
alignment, and other hits to this gene. In addition, P-element
information is found at this level. (MIDDLE & BOTTOM) Example
Homophila query using the keyword "neuropathy". The user enters
the key word and the database will return any human entry or Drosophila
gene description that contains the key word. In this case, there are several
human neuropathies listed, including a gene for peripheral neuropathy,
which is a transcription factor (Krox20). By clicking the "details"
button in this first window, one can examine the particular BLAST comparisons
of the human genes to Drosophila genes as well as the P-element
information. By scrolling down in this window, one can look at particular
alignments between the query sequence and its Drosophila matches.
In this case, the human Krox20 matches Drosophila stripe
gene most strongly in the DNA-binding domains, but also retains some overall
sequence similarity in other domains as can clearly be seen on the color
graphical alignment of similar sequences.
Courtesy Genome Research
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