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How to query the Homophila database. (TOP) Schematic of a Homophila query. The user enters the text query in the form of human disease name, Online Mendelian Inheritance in Man (OMIM) number, fly gene name, or keyword search through the human disease entry box. The database then opens a window with information on the disease name, and human and fly genes that match the key word query. The user then can examine the details of an individual human to Drosophila BLAST comparison to get more information on the specific BLAST score, alignment, and other hits to this gene. In addition, P-element information is found at this level. (MIDDLE & BOTTOM) Example Homophila query using the keyword "neuropathy". The user enters the key word and the database will return any human entry or Drosophila gene description that contains the key word. In this case, there are several human neuropathies listed, including a gene for peripheral neuropathy, which is a transcription factor (Krox20). By clicking the "details" button in this first window, one can examine the particular BLAST comparisons of the human genes to Drosophila genes as well as the P-element information. By scrolling down in this window, one can look at particular alignments between the query sequence and its Drosophila matches. In this case, the human Krox20 matches Drosophila stripe gene most strongly in the DNA-binding domains, but also retains some overall sequence similarity in other domains as can clearly be seen on the color graphical alignment of similar sequences.

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