Phenotypic studies of hABCA1 in transgenic mice A human BAC containing the structure gene and flanking regions of ABCA1 was introduced into mouse embryos to create several lines of transgenic mice. Panel A shows that the expression of hABCA1 in these mice mimics that of the endogenous mABCA1: in both cases ABCA1 is predominantly expressed in liver. It indicates that the human BAC contains sufficient sequence information needed for tissue specific expression of hABCA1 in mice. Panel B shows that an increase in the overall expression of ABCA1 in transgenics resulted in increased cholesterol efflux in macrophages. The increase in cholesterol efflux, however, does not seem to affect the plasma levels of HDL and total cholesterol as seen in Panel C.
Courtesy Jan-Fang Cheng