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Yeast proteome microarray developed
Edward R. Winstead

Researchers have created a novel microarray by assembling 5,800 yeast proteins on a glass slide. The microarray, or yeast proteome chip, was used to detect molecular interactions such as the binding of proteins on the chip to other proteins. Similar proteome chips can be prepared and used to monitor a range of biochemical activities, including protein-drug interactions, the researchers say.

Michael Snyder, of Yale University, and colleagues purified the protein products of 5,800 cloned yeast genes and printed them on glass slides. They tested the chips by screening for protein-protein interactions and protein-lipid interactions. The research appears in Science Express.

Snyder and colleagues determined that 13,000 protein samples could be spotted in half the area of a standard microscope slide with excellent resolution. "Using similar procedures, it is clearly possible to prepare protein arrays of 10-100,000 proteins for global proteome analysis in humans and other eucaryotes," they conclude.

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Zhu, H. et al. Global analysis of protein activities using proteome chips. Science Express (July 26, 2001).

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